Histone H2B antibody (AA 118-126)

Catalog No. ABIN211182

This anti-Histone H2B antibody is a Rabbit Polyclonal antibody detecting Histone H2B in WB. Suitable for Human.

Quick Overview for Histone H2B antibody (AA 118-126) (ABIN211182)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 118-126

Specificity: Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000 - 1:10000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 200 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Histone H2B

Target: Histone H2B