14-3-3 sigma/SFN antibody

Catalog No. ABIN211328

The Mouse Monoclonal anti-14-3-3 sigma/SFN antibody has been validated for WB and IP. It is suitable to detect 14-3-3 sigma/SFN in samples from Human, Mouse and Rat.

Quick Overview for 14-3-3 sigma/SFN antibody (ABIN211328)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-14-3-3 sigma/SFN Antibody

Specificity: Recognizes human 14-3-3 sigma, Mr 30kD. Does not cross-react with other 14-3-3 isoforms. Species cross-reactivity: rat and mouse.

Purification: Protein G purified

Immunogen: GST fusion protein corresponding to full-length human 14-3-3 sigma.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 μg/mL. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 0014-19. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.014 M phosphate buffer, 0.175 M sodium chloride,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN / Stratifin / 14-3-3 Sigma

Background: Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction