Src antibody

Catalog No. ABIN211401

The Mouse Monoclonal anti-Src antibody (ABIN211401) specifically detects Src in WB, ICC and IP. The antibody is reactive with Chicken samples.

Quick Overview for Src antibody (ABIN211401)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Src Antibody

Specificity: Recognizes and is specific only for chicken Src, 60kD. This antibody is excellent for distinguishing pp60src in cells transfected with v-src or c-src expression vectors.

Purification: Protein G purified

Immunogen: Chicken Src, purified from extracts of E. coli containing the plasmid plac-src.

Type of Immunogen: Protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ICC (10 μg/mL), IP, WB (1 - 5 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-5 μg/mL detected Src (60kD) in a RIPA lysate of chicken embryo fibroblasts (CEF). Immunoprecipitation: 1-2 μg immunoprecipitated Src using 250-500 μg of Src-transformed chicken cell extract, as reported by an independent laboratory. Immunocytochemistry: 10 μg/mL.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC

Background: Name/Gene ID: SRC
Subfamily: Src
Family: Protein Kinase

Synonyms: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling