EPH Receptor A2 antibody

Catalog No. ABIN211915

This Mouse Monoclonal antibody specifically detects EPH Receptor A2 in WB, IP, ICC and Func. It exhibits reactivity toward Human, Mouse, Rat, Dog and Cow.

Quick Overview for EPH Receptor A2 antibody (ABIN211915)

Target: EPH Receptor A2 (EPHA2)

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Product Details anti-EPH Receptor A2 Antibody

Specificity: Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.

Purification: Protein G purified

Immunogen: Native protein isolated by purification of phosphotyrosine-containing proteins.

Isotype: IgG1

Application Details

Application Notes: Approved: Func, ICC, IP, WB

Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EPHA2 / EPH Receptor A2

Background: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling