ERBB3 antibody (AA 1295-1323)

Catalog No. ABIN211920

This anti-ERBB3 antibody is a Mouse Monoclonal antibody detecting ERBB3 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for ERBB3 antibody (AA 1295-1323) (ABIN211920)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ERBB3 Antibody

Binding Specificity: AA 1295-1323

Specificity: Recognizes erbB-3 (185kD). Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2 Species cross-reactivity: Human. Species sequence Homology: Mouse, rat and bovine.

Purification: Protein A purified

Immunogen: Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. Epitope maps between aa1295-1323 of human erbB-3.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ICC, IHC (1:50 - 1:100), IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg antibody immunoprecipitates erbB-3 from human MDA and MCF-7 RIPA lysates. Immunohistochemistry: 1:50-1:100 dilution of a previous lot detected erbB-3 membrane staining in human normal and carcinoma breast tissue.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3 / ERBB3

Background: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

Gene ID: 2065

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway