IQGAP1 antibody (AA 863-1657)

Catalog No. ABIN211969

This Mouse Monoclonal antibody specifically detects IQGAP1 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for IQGAP1 antibody (AA 863-1657) (ABIN211969)

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IQGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IQGAP1 Antibody

Binding Specificity: AA 863-1657

Specificity: Recognizes human IQGAP1 at 185kD. Does not recognize IQGAP2.

Purification: Protein G purified

Immunogen: A Glu-Glu tagged C-terminal fragment corresponding to aa863-1657 of human IQGAP1 purified from baculovirus infected insect cells.

Type of Immunogen: Cells

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated IQGAP1 from 500 μg of a HepG2 RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Alternative Name: IQGAP1

Background: Name/Gene ID: IQGAP1
Family: Protease Regulatory

Synonyms: IQGAP1, HUMORFA01, p195, KIAA0051, RasGAP-like with IQ motifs, SAR1

Gene ID: 8826

UniProt: P46940

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2