PTK2B antibody (AA 726-863)

Catalog No. ABIN212775

Product Details anti-PTK2B Antibody

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Binding Specificity: AA 726-863

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes Pyk2 at 116kD in mouse brain cytosol. Also detects a band at 40kD, which may be a degradation product. Does not cross-react with FAK. Species cross-reactivity: Human and mouse.

Purification: Protein A purified

Immunogen: GST fusion protein containing aa726-863 of human Pyk2.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit (HRP) secondary antibody and a chemiluminescence detection system. Pyk2 has a MW of approximately 116kD as calculated from the amino acid sequence deduced from the complete human cDNA. Immunoprecipitation: 4 μg detected Pyk2 from 500 μg of mouse brain cytosolic RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PTK2B / PYK2 (PTK2B Products)

Synonyms: CADTK antibody, CAKB antibody, FADK2 antibody, FAK2 antibody, PKB antibody, PTK antibody, PYK2 antibody, RAFTK antibody, CAKbeta antibody, E430023O05Rik antibody, Raftk antibody, Pyk2 antibody, fak1b antibody, ptk2.2 antibody, ptk2b antibody, si:ch1073-355e8.1 antibody, protein tyrosine kinase 2 beta antibody, PTK2 protein tyrosine kinase 2 beta antibody, protein tyrosine kinase 2aa antibody, PTK2B antibody, Ptk2b antibody, ptk2aa antibody

Background: Name/Gene ID: PTK2B
Subfamily: Focal Adhesion Kinase
Family: Protein Kinase

Synonyms: PTK2B, CADTK, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, Focal adhesion kinase 2, FRNK, FAK2, Proline-rich tyrosine kinase 2, Protein-tyrosine kinase 2-beta, Protein kinase B, Protein tyrosine kinase 2 beta, PYK2, RAFTK, CAK-beta, PKB, PTK

Gene ID: 2185

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling