Paxillin antibody

Catalog No. ABIN212824

Product Details anti-Paxillin Antibody

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.

Purification: Protein G purified

Immunogen: GST fusion protein containing full-length human paxillin.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: PXN / Paxillin (PXN Products)

Synonyms: AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody

Background: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

Gene ID: 5829

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling