Vimentin antibody

Catalog No. ABIN213302

The Mouse Monoclonal anti-Vimentin antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect Vimentin in samples from Human, Rat, Pig, Cow, Chicken, Monkey, Dog and Cat.

Quick Overview for Vimentin antibody (ABIN213302)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Vimentin Antibody

Specificity: Recognizes a 57-60kD protein, human Vimentin. Shows no cross-reactivity with other closely related intermediate filament proteins including desmin and GFAP. Species cross-reactivity: monkey, bovine, canine, feline, chicken and rat. Does not recognize mouse.

Purification: Purified

Immunogen: Purified vimentin from porcine eye lens.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (5 - 10 μg/mL), IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 5-10 μg/mL, Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity. Immunocytochemistry: 5-10 μg/mL. Positive control: Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis