Serotonin Receptor 1E antibody (Cytoplasmic Domain)

Catalog No. ABIN213509

The Rabbit Polyclonal anti-Serotonin Receptor 1E antibody has been validated for ELISA, IHC (p) and IHC. It is suitable to detect Serotonin Receptor 1E in samples from Human, Horse, Guinea Pig, Monkey and Bat.

Quick Overview for Serotonin Receptor 1E antibody (Cytoplasmic Domain) (ABIN213509)

Target: Serotonin Receptor 1E (HTR1E)

Reactivity: Human, Horse, Guinea Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 1E antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-Serotonin Receptor 1E Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Bat, Elephant, Horse, Guinea pig (100%) Orangutan, Gibbon, Bovine (95%) Panda (89%) Opossum (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Bat, Elephant, Horse, Guinea pig (100%), Orangutan, Gibbon, Bovine (95%), Panda (89%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (16 - 24 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 16-24 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Serotonin Receptor 1E

Target: Serotonin Receptor 1E (HTR1E)

Alternative Name: HTR1E / 5-HT1E Receptor

Background: Name/Gene ID: HTR1E
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR1E, 5-HT1E, 5-HT1e receptor, 5-HT-1E, 5HT1E Receptor, HT1e receptor, S31, Serotonin 5-HT-1e receptor, Serotonin 1e receptor, Serotonin receptor 1E

Gene ID: 3354

Pathways: JAK-STAT Signaling, cAMP Metabolic Process