Aurora Kinase B antibody (AA 227-238)

Catalog No. ABIN214031

The Rabbit Polyclonal anti-Aurora Kinase B antibody is suitable to detect Aurora Kinase B in samples from Human. It has been validated for IHC, WB, ELISA, IF and IHC (p).

Quick Overview for Aurora Kinase B antibody (AA 227-238) (ABIN214031)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 227-238

Specificity: Aa 227-238 of Human Aurora Kinase B protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human AURKB / Aurora-B.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB / Aurora-B

Background: Name/Gene ID: AURKB
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKB, AIRK2, AIK2, Aurora kinase B, Aurora kinase B-Sv1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, ARK-2, ARK2, Aurkb-sv1, Aurkb-sv2, Aurora 1, Aurora-B, AIM-1, IPL1, STK-1, STK5, PPP1R48, AurB, Aurora B, Aurora kinase B-Sv2, Aurora-1, Aurora1, Serine/threonine kinase 12, STK1, STK12

Gene ID: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades