RIF1 antibody (AA 2406-2419)

Catalog No. ABIN214034

The Rabbit Polyclonal anti-RIF1 antibody is suitable to detect RIF1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for RIF1 antibody (AA 2406-2419) (ABIN214034)

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RIF1 Antibody

Binding Specificity: AA 2406-2419

Specificity: Rif1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from mouse RIF1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:40000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for RIF1

Target: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Alternative Name: RIF1

Background: Name/Gene ID: RIF1

Synonyms: RIF1

Gene ID: 55183

UniProt: Q5UIP0

Pathways: Stem Cell Maintenance