PLK1 antibody (pThr210)

Catalog No. ABIN214228

This Mouse Monoclonal antibody specifically detects PLK1 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PLK1 antibody (pThr210) (ABIN214228)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2A3

Product Details anti-PLK1 Antibody

Binding Specificity: pThr210

Specificity: Modified peptide

Purification: Affinity purified

Immunogen: Synthetic peptide from human PLK1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide at 0.5 mg/mL. Sourced from tissue culture supernatant.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 / PLK-1

Background: Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase

Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase