CARD9 antibody (AA 521-536)

Catalog No. ABIN214431

This anti-CARD9 antibody is a Rabbit Polyclonal antibody detecting CARD9 in WB, IHC, ICC and IHC (p). Suitable for Human and Monkey.

Quick Overview for CARD9 antibody (AA 521-536) (ABIN214431)

Target: CARD9 (Caspase Recruitment Domain Family, Member 9 (CARD9))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CARD9 Antibody

Binding Specificity: AA 521-536

Brand: IHC-plus™

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Mouse, Elephant, Panda, Dog, Horse, Opossum (94%) Monkey, Rat (88%) Bovine, Turkey, Chicken (81%).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CARD9

Target: CARD9 (Caspase Recruitment Domain Family, Member 9 (CARD9))

Alternative Name: CARD9

Background: Name/Gene ID: CARD9
Family: Apoptosis

Synonyms: CARD9, CANDF2, HCARD9

Gene ID: 64170

UniProt: Q9H257

Pathways: Activation of Innate immune Response