PLK1 antibody (pThr210)

Catalog No. ABIN214445

The Rabbit Polyclonal anti-PLK1 antibody is suitable to detect PLK1 in samples from Human. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for PLK1 antibody (pThr210) (ABIN214445)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLK1 Antibody

Binding Specificity: pThr210

Specificity: Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

Purification: Immunoaffinity purified

Immunogen:

Type of Immunogen: Native protein

Application Details

Application Notes: Approved: ELISA (1:3000 - 1:12000), IHC, IHC-P (10 μg/mL), WB (1:200 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.Western Blot: It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 / PLK-1

Background: Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase

Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase