NLRC4 antibody (C-Term)

Catalog No. ABIN214456

This Rabbit Polyclonal antibody specifically detects NLRC4 in WB, IHC, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NLRC4 antibody (C-Term) (ABIN214456)

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NLRC4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Alternative Name: IPAF / NLRC4

Background: Name/Gene ID: NLRC4
Family: Apoptosis

Synonyms: NLRC4, CARD12, CLANA, CLANB, CLANC, CLAND, CLAN, CLR2.1, Clan protein, CLAN1, Ice protease-activating factor, NOD-like receptor C4, ICE-protease activating factor, IPAF

Gene ID: 58484

UniProt: Q9NPP4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome