RRM2B antibody (AA 2-17)

Catalog No. ABIN214469

The Rabbit Polyclonal anti-RRM2B antibody is suitable to detect RRM2B in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for RRM2B antibody (AA 2-17) (ABIN214469)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RRM2B Antibody

Binding Specificity: AA 2-17

Specificity: At least six isoforms of p53R2 are known to exist, this antibody will detect all but the largest isoform.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan (100%) Monkey, Marmoset (94%) Horse (81%).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Alternative Name: RRM2B

Background: Name/Gene ID: RRM2B
Family: RIBONUCLEOTIDE REDUCTASE

Synonyms: RRM2B, MTDPS8A, MTDPS8B, p53R2, PEOA5

Gene ID: 50484

UniProt: Q7LG56

Pathways: p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling