SGK1 antibody (AA 419-431)

Catalog No. ABIN214577

The Rabbit Polyclonal anti-SGK1 antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect SGK1 in samples from Human, Mouse and Rat.

Quick Overview for SGK1 antibody (AA 419-431) (ABIN214577)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SGK1 Antibody

Binding Specificity: AA 419-431

Brand: IHC-plus™

Specificity: Amino acids 419-431 of human SGK -1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human SGK1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:16000), IHC, IHC-P (10 - 20 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10-20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK1 / SGK

Background: Name/Gene ID: SGK1
Subfamily: SGK
Family: Protein Kinase

Synonyms: SGK1, Hsgk, Hsgk1, SGK, Sgk1 variant i3

Gene ID: 6446

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway