alpha Tubulin antibody (AA 427-441)

Catalog No. ABIN214603

This Rabbit Polyclonal antibody specifically detects alpha Tubulin in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow and Chicken.

Quick Overview for alpha Tubulin antibody (AA 427-441) (ABIN214603)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 427-441

Specificity: Amino acids 427-441 of Human alpha Tubulin.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human Tubulin, Alpha 1 (TUBA1).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Pathways: Microtubule Dynamics