AGRN antibody (C-Term)

Catalog No. ABIN214988

The Mouse Monoclonal anti-AGRN antibody has been validated for WB and ICC. It is suitable to detect AGRN in samples from Rat and Mouse.

Quick Overview for AGRN antibody (C-Term) (ABIN214988)

Target: AGRN (Agrin (AGRN))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-AGRN Antibody

Binding Specificity: C-Term

Specificity: Detects all isoforms of agrin in samples from mouse and rat. Epitope is located near the 1st EGF-like repeat. Recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants. Species Reactivity: mouse, rat.

Purification: Protein G purified

Immunogen: Recombinant rat agrin, C-terminal construct.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, WB

Usage: Western Blot (Colorimetric): 4 μg/mL. Western Blot (ECL): 4 μg/mL. Immunocytochemistry: 1:50.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRN / Agrin

Background: Name/Gene ID: AGRN

Synonyms: AGRN, Agrin, Agrin proteoglycan

Gene ID: 375790

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development