HSP27 antibody
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- Target See all HSP27 (HSPB1) Antibodies
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Reactivity
- Human, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HSP27 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.
- Purification
- Protein G purified
- Immunogen
- Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.
- Isotype
- IgG1
- Top Product
- Discover our top product HSPB1 Primary Antibody
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- Application Notes
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Approved: ELISA, ICC (10 μg/mL), IF, IHC (5 μg/mL), IP (12.5 μg/mL), WB
Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. Western: Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL. Immunohistochemistry: 5 μg/mL. Immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 0.1 mM PMSF, 50 % glycerol.
- Preservative
- PMSF
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Alternative Name
- HSPB1 / HSP27 (HSPB1 Products)
- Synonyms
- CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
- Background
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Name/Gene ID: HSPB1
Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27 kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067 - Gene ID
- 3315
- UniProt
- P04792
- Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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