ATP1A1 antibody

Catalog No. ABIN215644

This Mouse Monoclonal antibody specifically detects ATP1A1 in WB, IHC and IP. It exhibits reactivity toward Human, Rat, Mouse, Pig, Dog, Monkey and Xenopus laevis.

Quick Overview for ATP1A1 antibody (ABIN215644)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human, Rat, Mouse, Pig, Dog, Monkey, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ATP1A1 Antibody

Specificity: Recognizes rabbit Na+/K+ ATPase alpha-1 subunit. Species cross-reactivity: Human, rabbit, monkey, rat, mouse, lamb, canine, porcine and Xenopus.

Purification: Protein G purified

Immunogen: Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IP, WB (0.05 - 0.1 μg/mL)

Usage: Western Blot: 0.05-0.1 μg/mL detected the a-1 subunit in 20 μg of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05 % Tween-20 in PBS for 15 minutes.Immunohistochemistry: 1-10 μg/mL on ice-cold methanol-fixed tissue.Immunoprecipitation: 2 μg immunoprecipitated the a-1 subunit from rat brain microsomal preparations.Optimal dilutions to be determined by the researcher.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1

Background: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process