Dopamine d2 Receptor antibody (3rd Cytoplasmic Domain)

Catalog No. ABIN215781

The Rabbit Polyclonal anti-Dopamine d2 Receptor antibody (ABIN215781) specifically detects Dopamine d2 Receptor in WB, IHC, ELISA and IP. The antibody is reactive with Human, Rat, Mouse and Monkey samples.

Quick Overview for Dopamine d2 Receptor antibody (3rd Cytoplasmic Domain) (ABIN215781)

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine d2 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-Dopamine d2 Receptor Antibody

Binding Specificity: 3rd Cytoplasmic Domain

Specificity: Specific for Dopamine Receptor D2 short and long form. Species cross-reactivity: Monkey, mouse and rat.

Purification: Affinity purified

Immunogen: A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic loop #3. The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5). Species sequence Homology: Bovine, canine - 100%, Xenopus - 50%.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC, IP (1 - 10 μg/mL), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and ELISA. Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10 μg of human brain lysates. Immunohistochemistry (Paraformaldehyde fixed sections): 2-10 μg/mL. Immunoprecipitation: 1-10 μg/mL. Using 100-400 μg of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms. ELISA: 0.5-1.0 μg/mL. Positive control: Monkey brain tissue, rat brain lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Dopamine d2 Receptor

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Alternative Name: DRD2 / Dopamine Receptor D2

Background: Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2

Gene ID: 1813

UniProt: P14416

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity