Dopamine d2 Receptor antibody (3rd Cytoplasmic Domain)

Catalog No. ABIN215782

This Rabbit Polyclonal antibody specifically detects Dopamine d2 Receptor in WB and ELISA. It exhibits reactivity toward Rat and Dog.

Quick Overview for Dopamine d2 Receptor antibody (3rd Cytoplasmic Domain) (ABIN215782)

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Reactivity: Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine d2 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Dopamine d2 Receptor Antibody

Binding Specificity: 3rd Cytoplasmic Domain

Specificity: Recognizes rat Dopamine Receptor D2 long form. Recognizes canine D2R. Species sequence Homology: Human, bovine, and canine: 96 % , Xenopus: 64 %.

Purification: Immunoaffinity purified

Immunogen: A 27 amino acid peptide sequence from the rat D2 long form within the cytoplasmic loop #3. No significant homology with other receptors (D1, D2 short, and D3-D5).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 10 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot: 1-10 μg/mL (Chemiluminescence technique). The predicted size of D2L form is approximately 50kD. However, additional bands at approximately 60 and 100kD, and others are typically seen in various tissues. Approximately 100-400 μg of membrane protein/lane should be loaded to visualize various bands. ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide/well).

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Dopamine d2 Receptor

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Alternative Name: DRD2 / Dopamine Receptor D2

Background: Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2

Gene ID: 1813

UniProt: P14416

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity