CD14 antibody (FITC)

Catalog No. ABIN217703

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS. It is suitable to detect CD14 in samples from Human, Cynomolgus and Rhesus Monkey.

Quick Overview for CD14 antibody (FITC) (ABIN217703)

Target: CD14

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-CD14 Antibody (FITC)

Specificity: Recognizes a cell surface antigen of 55 kD, known as CD14. The CD14 Molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 Molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 Molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 Reacts with Kupffer cells and sinus lining cells on the liver.

Purification: Protein G purified

Immunogen: Human Thymocytes followed by peripheral blood mononuclear cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:10)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Name/Gene ID: CD14

Synonyms: CD14, CD14 Molecule, CD14 antigen

Gene ID: 929

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades