POLE antibody (AA 101-200)

Catalog No. ABIN2178556

This Rabbit Polyclonal antibody specifically detects POLE in ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLE antibody (AA 101-200) (ABIN2178556)

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-POLE Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase epsilon subunit 2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POLE

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Alternative Name: DNA polymerase epsilon

Background:

Synonyms: DPE2, DNA polymerase epsilon subunit 2, DNA polymerase II subunit 2, DNA polymerase epsilon subunit B, POLE2

Background: Participates in DNA repair and in chromosomal DNA replication.

Gene ID: 5427

UniProt: P56282

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA