POLE antibody (AA 1230-1310)

Catalog No. ABIN7223256

The Rabbit Polyclonal anti-POLE antibody (ABIN7223256) specifically detects POLE in ELISA, IHC and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for POLE antibody (AA 1230-1310) (ABIN7223256)

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-POLE Antibody

Binding Specificity: AA 1230-1310

Purpose: Rabbit Anti-DNA pol ε A Polyclonal Antibody

Specificity: DNA pol ε A Polyclonal Antibody detects endogenous levels of DNA pol ε A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DNA pol epsilon A at AA range: 1230-1310

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLE

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Alternative Name: DNA pol epsilon A

Background: POLE, POLE1, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit APOLE (DNA polymerase epsilon, catalytic subunit) encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in POLE have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature.

Gene ID: 5426

UniProt: Q07864

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA