IL-13 antibody

Catalog No. ABIN218186

The Rat Monoclonal anti-IL-13 antibody has been validated for WB, ELISA, FACS, Neut and IF. It is suitable to detect IL-13 in samples from Human.

Quick Overview for IL-13 antibody (ABIN218186)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF)

Product Details anti-IL-13 Antibody

Specificity: Recognizes human Interleukin-13 (IL-13).

Purification: Purified

Immunogen: COS-expressed recombinant human IL-13.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo, IF, Neut, SwELISA, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-13-positive cells within mixed cell populations. Recombinant human IL-13, or Rat IgG1 should be used as a specificity control. Western Blot: Useful for Western blot applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing human IL-13 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservative added.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background: Name/Gene ID: IL13
Family: Hormone

Synonyms: IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13

Gene ID: 3596

UniProt: P35225

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport