NEK9 antibody

Catalog No. ABIN218339

The Rabbit Polyclonal anti-NEK9 antibody is suitable to detect NEK9 in samples from Human. It has been validated for WB, IHC, IP and IHC (p).

Quick Overview for NEK9 antibody (ABIN218339)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NEK9 Antibody

Specificity: Partial recombinant protein, responding to the middle region of NEK-9

Purification: Immunoaffinity purified

Immunogen: Recombinant human NEK9.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:50), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Alternative Name: NEK9

Background: Name/Gene ID: NEK9
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9

Gene ID: 91754

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection