IL-6 antibody

Catalog No. ABIN2191862

The Mouse Monoclonal anti-IL-6 antibody has been validated for WB, IP, Neut and IA. It is suitable to detect IL-6 in samples from Human.

Quick Overview for IL-6 antibody (ABIN2191862)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut), Immunoassay (IA)

Clone: 5E1

Product Details anti-IL-6 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions, starting with undiluted material. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-6 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: Interleukin-6

Background: The antibody reacts with human native and recombinant IL-6 as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant IL-6 as determined with the B9 cell bioassay. The antibody cross reacts with rhesus and cynomolgus natural IL6.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome