TLR4 antibody

Catalog No. ABIN2191937

The Mouse Monoclonal anti-TLR4 antibody has been validated for FACS and Func. It is suitable to detect TLR4 in samples from Human. There are 6+ publications available.

Quick Overview for TLR4 antibody (ABIN2191937)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: HTA125

Product Details anti-TLR4 Antibody

Cross-Reactivity (Details): Cross reactivity: Canine : Yes, Cynomolgus monkey : Yes, Rhesus monkey : Yes, Marmoset monkey : Yes

Sterility: 0.2 μm filtered

Isotype: IgG2a

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Macrophages control Negative HEK293 cells control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TLR4 antibody (ABIN2191937)

Brüll, Mensink, van den Hurk, Duijvestijn, Plat: "TLR2 activation is essential to induce a Th1 shift in human peripheral blood mononuclear cells by plant stanols and plant sterols." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 2951-8, (2010) (PubMed).

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, (2008) (PubMed).

Triantafilou, Morath, Mackie, Hartung, Triantafilou: "Lateral diffusion of Toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane." in: Journal of cell science, Vol. 117, Issue Pt 17, pp. 4007-14, (2004) (PubMed).

Walton, Hsieh, Gharavi, Wang, Wang, Yeh, Cole, Berliner et al.: "Receptors involved in the oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine-mediated synthesis of interleukin-8. A role for Toll-like receptor 4 and a ..." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29661-6, (2003) (PubMed).

Tabeta, Yamazaki, Akashi, Miyake, Kumada, Umemoto, Yoshie: "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts." in: Infection and immunity, Vol. 68, Issue 6, pp. 3731-5, (2000) (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: Toll-Like Receptor 4

Background: Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB- dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4, CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways, one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro-inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR- domaincontaining adaptor protein inducing interferon-β) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2. Aliases TLR4, CD284, ARMD10 Immunogen BALB/c mice were immunized with the Ba/F3 line expressing TLR4.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins