C5 antibody

Catalog No. ABIN2191947

Product Details anti-C5 Antibody

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is un-conjugated

Application: Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: 561

Application Details

Application Notes: The dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-C5 antibody (ABIN2191947)

Fung, Lu, Fure, Sun, Sun, Shi, Dou, Su, Swanson, Mollnes: "Pre-neutralization of C5a-mediated effects by the monoclonal antibody 137-26 reacting with the C5a moiety of native C5 without preventing C5 cleavage." in: Clinical and experimental immunology, Vol. 133, Issue 2, pp. 160-9, (2003) (PubMed).

Mollnes, Brekke, Fung, Fure, Christiansen, Bergseth, Videm, Lappegård, Köhl, Lambris: "Essential role of the C5a receptor in E coli-induced oxidative burst and phagocytosis revealed by a novel lepirudin-based human whole blood model of inflammation." in: Blood, Vol. 100, Issue 5, pp. 1869-77, (2002) (PubMed).

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: c5/c5a (C5 Products)

Synonyms: C5 antibody, LOC565774 antibody, C5a antibody, C5b antibody, CPAMD4 antibody, Hc antibody, RGD1561905 antibody, He antibody, complement C5 antibody, complement component 5 antibody, hemolytic complement antibody, C5 antibody, c5 antibody, Hc antibody

Background: Monoclonal antibody 561 recognizes the N-terminus of C5. The antibody reacts both with intact C5 (190 kDa) as with C5a (115 kDa). The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b, resulting in activation of the lytic pahway C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis. Immunogen Human C5/C5a

Pathways: Complement System, Carbohydrate Homeostasis