C3AR1 antibody

Catalog No. ABIN2192116

This Mouse Monoclonal antibody specifically detects C3AR1 in FACS and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for C3AR1 antibody (ABIN2192116)

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3AR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 74 (rC3aRZ1)

Product Details anti-C3AR1 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For flow cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for C3AR1

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Background: The monoclonal antibody 74 reacts with the rat receptor for C3a. The monoclonal antibody 74 is in the literature also referred to as clone rC3aRZ1. In the course of complement activation the C3a receptor functions as the cell surface receptor for the anaphylatoxin C3a, the C-terminal 77 amino acid cleavage product of the alpha chain of C3, but not for C3a-desArg. All cellular responses to C3a are specifically mediated by interactions with the membrane bound C3a receptor, a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 54 kDa C3a receptor is widely distributed in myeloid cells, peripheral tissues and the central nervous system. Expression of C3aR has been demonstrated on neutrophils, monocytes, eosinophils, astrocytes, neurons and glial cells and is increased in inflammatory conditions. No C3aR expression was detectable on lymphocytes and on tonsillar B cells even after stimulation.

Pathways: Complement System