C-JUN antibody (pSer73)
Quick Overview for C-JUN antibody (pSer73) (ABIN220003)
Target
See all C-JUN (JUN) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer73
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Specificity
- Detects endogenous levels of c-Jun only when activated by phosphorylation at Ser73. Also recognizes phosphorylation of JunD at Ser100. Species cross-reactivity: Human, mouse and rat.
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser73 of human c-Jun.
Type of Immunogen: Synthetic phospho-peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA, IF, IHC, IHC-P, WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1:1000. Immunofluorescence (IF-IC): 1:100. Immunohistochemistry (Paraffin): 1:50. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
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Alternative Name
- JUN / c-Jun
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Background
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Name/Gene ID: JUN
Family: Transcription factor
Synonyms: JUN, Activator protein 1, AP-1, C-Jun, Jun proto-oncogene, p39, Jun oncogene, Proto-oncogene c-Jun, AP1, Enhancer-binding protein AP1, G0s7, Transcription factor AP-1, v-jun -
Gene ID
- 3725
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UniProt
- P05412
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Pathways
- MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
Target
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