MAP2K4 antibody (pThr261)

Catalog No. ABIN220428

This anti-MAP2K4 antibody is a Rabbit Polyclonal antibody detecting MAP2K4 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for MAP2K4 antibody (pThr261) (ABIN220428)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP2K4 Antibody

Binding Specificity: pThr261

Specificity: Detects endogenous levels of human SEK1/ MKK4 protein only when phosphorylated at threonine 261. Does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. Species cross-reactivity: Mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr261 of human SEK1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:2000)

Usage: Western Blot: 1:2000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: MAP2K4 / MKK4

Background: Name/Gene ID: MAP2K4
Subfamily: MAP2K
Family: Protein Kinase

Synonyms: MAP2K4, JNK activating kinase 1, JNK-activated kinase 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MAPKK4, JNKK1, PRKMK4, SAPKK1, SEK1, MEK4, MKK4, SAPK/ERK kinase 1, SERK1, JNKK, MAP kinase kinase 4, MAPKK 4, MEK 4, SAPK kinase 1, SAPKK-1, SKK1

Gene ID: 6416

UniProt: P45985

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling