JNK antibody (pThr183, Tyr185)

Catalog No. ABIN220911

This anti-JNK antibody is a Rabbit Polyclonal antibody detecting JNK in WB, ELISA, IHC, IP and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for JNK antibody (pThr183, Tyr185) (ABIN220911)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JNK Antibody

Binding Specificity: pThr183, Tyr185

Specificity: Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species cross-reactivity: mouse, monkey, hamster, bovine, rat and Drosophila. Species sequence homology: Xenopus.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (1:75), IP (1:200), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:200. Immunohistochemistry (Paraffin): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 / JNK1 / JNK

Background: Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins