JNK antibody (N-Term)

Catalog No. ABIN220913

The Rabbit Polyclonal anti-JNK antibody has been validated for WB, FACS and ICC. It is suitable to detect JNK in samples from Human, Mouse and Rat.

Quick Overview for JNK antibody (N-Term) (ABIN220913)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-JNK Antibody

Binding Specificity: N-Term

Specificity: Detects endogenous levels of total SAPK/JNK protein. Species cross-reactivity: Human, mouse, rat and zebrafish.

Purification: Protein A purified

Immunogen: Fusion protein of human JNK2 and GST.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:25), ICC, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunocytochemistry and Flow Cytometry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (ABC): 1:200. Flow Cytometry: 1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 / JNK1 / JNK

Background: Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins