NEDD8 antibody (N-Term)

Catalog No. ABIN221543

The Rabbit Polyclonal anti-NEDD8 antibody has been validated for WB, IHC, IF, IHC (p), FACS and IP. It is suitable to detect NEDD8 in samples from Human, Mouse, Rat, Monkey, Cow and Xenopus laevis.

Quick Overview for NEDD8 antibody (N-Term) (ABIN221543)

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-NEDD8 Antibody

Binding Specificity: N-Term

Specificity: Detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15. Species cross-reactivity: Bovine, human, monkey, mouse, rat and Xenopus.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids at the amino terminus of human NEDD8 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Flo (1:200), IF (1:200), IHC, IHC-P, IP (1:25), WB (1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Flow Cytometry. Western Blot 1:1000. Immunoprecipitation 1:25. Immunofluorescence (IF-IC) 1:200. Flow Cytometry: 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, less than 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8

Background: Name/Gene ID: NEDD8

Synonyms: NEDD8, NEDD-8, Neddylin, Ubiquitin-like protein Nedd8

Gene ID: 4738

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway