MSH2 antibody (C-Term)

Catalog No. ABIN226178

The Mouse Monoclonal anti-MSH2 antibody has been validated for WB, IHC, IP, IHC (p) and IHC (fro). It is suitable to detect MSH2 in samples from Human and Mouse.

Quick Overview for MSH2 antibody (C-Term) (ABIN226178)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MSH2 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human and mouse MSH2 proteins (~100kD).

Purification: Protein A purified

Immunogen: A carboxy-terminal fragment of the human MSH2 protein.

Type of Immunogen: Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-Fr (2 μg/mL), IHC-P (2 μg/mL), IP, WB (0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot (chemiluminescence): 0.2 μg/mL. Immunoprecipitation: 1 μg/sample. Immunohistochemistry (Frozen): 2 μg/mL. Immunohistochemistry (Paraffin): 2 μg/mL (staining of formalin fixed, paraffin embedded tissue requires Heat induced epitope retrieval (HIER) pretreatment). Positive control: Immunoprecipitation and Western Blot: HCT116 or SW480 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: Name/Gene ID: MSH2

Synonyms: MSH2, COCA1, FCC1, HNPCC, HNPCC1, MutS protein homolog 2, HMSH2, LCFS2

Gene ID: 4436

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response