GM-CSF antibody

Catalog No. ABIN226520

The Rat Monoclonal anti-GM-CSF antibody is suitable to detect GM-CSF in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for GM-CSF antibody (ABIN226520)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GM-CSF Antibody

Specificity: Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).

Purification: Purified

Immunogen: Yeast-expressed recombinant mouse GM-CSF.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, WB

Usage: ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels.1,3-5 Biotinylated antibody should be paired with purified antibody (Cat. Western Blot: The purified antibody is also useful for immunoprecipitation4 and Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse GM-CSF under reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM borate buffered saline,  pH 8.0. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2 / GMCSF / GM-CSF

Background: Name/Gene ID: CSF2

Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin