GM-CSF antibody (Biotin)

Catalog No. ABIN226521

The Biotin-conjugated Rat Monoclonal anti-GM-CSF antibody (ABIN226521) specifically detects GM-CSF in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for GM-CSF antibody (Biotin) (ABIN226521)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-GM-CSF Antibody (Biotin)

Specificity: Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).

Purification: Purified

Immunogen: Yeast-expressed recombinant mouse GM-CSF.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse GM-CSF under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2 / GMCSF / GM-CSF

Background: Name/Gene ID: CSF2

Synonyms: CSF2, Colony-stimulating factor, CSF, GM-CSF, Sargramostim, Molgramostin, GMCSF

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin