Cyclin E1 antibody (pThr62)

Catalog No. ABIN227700

This anti-Cyclin E1 antibody is a Rabbit Polyclonal antibody detecting Cyclin E1 in WB, IHC, FACS, IHC (p) and IP. Suitable for Human.

Quick Overview for Cyclin E1 antibody (pThr62) (ABIN227700)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Cyclin E1 Antibody

Binding Specificity: pThr62

Specificity: Detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1). Species cross-reactivity: Human.

Purification: Immunoaffinity purified

Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr62 of human cyclin E.

Type of Immunogen: Synthetic phospho-peptide

Application Details

Application Notes: Approved: Flo (1:25), IHC, IHC-P (1:100), IP (1:100), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Flow Cytometry: 1:25. Western Blot: 1:1000. Immunohistochemistry: (Paraffin) 1:100. Citrate/TBST. Immunoprecipitation: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1 / Cyclin E1

Background: Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases