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Histone H2B antibody (acLys20)

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB, ELISA, IHC, ICC, IHC (p) and IP. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN228337

Quick Overview for Histone H2B antibody (acLys20) (ABIN228337)

Target

See all Histone H2B Antibodies
Histone H2B

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone H2B antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • Binding Specificity

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    acLys20

    Specificity

    Acetyl-Histone H2B (Lys20) Antibody detects histone H2B only when acetylated at Lys20. Does not cross-react with other acetylated histones. Species cross-reactivity: Arabidopsis, mouse and rat. Note: Adherence to the attached protocol is extremely important.

    Purification

    Protein A purified

    Immunogen

    Synthetic acetylated peptide (KLH coupled) corresponding to sequence surrounding Lys20 of human histone H2b.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: ELISA, ICC, IHC, IHC-P, IP (1:150), WB (1:1000)

    Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Paraffin sections) and Immunocytochemistry (ABC detection method). Western Blot: 1:1000. Immunoprecipitation: 1:150. Immunohistochemistry (Paraffin): 1:150. Immunocytochemistry (ABC): 1:150.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

    Preservative

    Without preservative
  • Target

    Histone H2B
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