KAT2B antibody (AA 817-832)

Catalog No. ABIN229084

This Rabbit Polyclonal antibody specifically detects KAT2B in WB and IP. It exhibits reactivity toward Human, Mouse, Dog, Cow, Horse, Monkey and Bat.

Quick Overview for KAT2B antibody (AA 817-832) (ABIN229084)

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Reactivity: Human, Mouse, Dog, Cow, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAT2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-KAT2B Antibody

Binding Specificity: AA 817-832

Specificity: Recognizes human and canine PCAF. Species cross-reactivity: Predicted to react with mouse and chicken due to sequence homology.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%) Rat, Opossum, Platypus, Xenopus (94%) Chicken, Lizard, Zebrafish (88%) Stickleback, Pufferfish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse (100%), Rat, Opossum, Platypus, Xenopus (94%), Chicken, Lizard, Zebrafish (88%), Stickleback, Pufferfish (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (1:500)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500, Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band (s) may appear. Staining of PCAF band (s) by immunoblotting is inhibited by the PCAF immunizing peptide. Immunoprecipitation: 5 μg immunoprecipitates PCAF from RIPA lysate with ~4x10^7 human acute T cell leukemia Jurkat cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 15 mM sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Alternative Name: KAT2B / PCAF

Background: Name/Gene ID: KAT2B

Synonyms: KAT2B, CREBBP-associated factor, GCN5, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, Lysine acetyltransferase 2B, Histone acetylase PCAF, PCAF, p300/CBP-associated factor, CAF, GCN5L

Gene ID: 8850

UniProt: Q92831

Pathways: p53 Signaling, Regulation of Carbohydrate Metabolic Process