PKC delta antibody (pSer643, Ser676)

Catalog No. ABIN229164

This anti-PKC delta antibody is a Rabbit Polyclonal antibody detecting PKC delta in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for PKC delta antibody (pSer643, Ser676) (ABIN229164)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC delta Antibody

Binding Specificity: pSer643, Ser676

Specificity: Detects endogenous levels of rat PKC delta only when phosphorylated at serines 643 and676 of rat PKC theta. Does not cross-react with the phosphorylated PKC isoforms alpha, beta, gamma or epsilon. Species cross-reactivity: Human and monkey. Predicted to cross-react with mouse based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser643 of rat PKC delta.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:1000)

Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PRKCD / PKC-Delta

Background: Name/Gene ID: PRKCD
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCD, MAY1, NPKC-delta, PKCD, Protein kinase C delta type, Protein kinase C delta VIII, Protein kinase C, delta, Pkcdelta, PKC delta, Protein kinase c delta, Tyrosine-protein kinase PRKCD

Gene ID: 5580

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism