PKC delta antibody (pSer643, Ser676)

Catalog No. ABIN229164

The Rabbit Polyclonal anti-PKC delta antibody (ABIN229164) specifically detects PKC delta in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for PKC delta antibody (pSer643, Ser676) (ABIN229164)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC delta Antibody

Binding Specificity: pSer643, Ser676

Specificity: Detects endogenous levels of rat PKC delta only when phosphorylated at serines 643 and676 of rat PKC theta. Does not cross-react with the phosphorylated PKC isoforms alpha, beta, gamma or epsilon. Species cross-reactivity: Human and monkey. Predicted to cross-react with mouse based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser643 of rat PKC delta.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:1000)

Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PRKCD / PKC-Delta

Background: Name/Gene ID: PRKCD
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCD, MAY1, NPKC-delta, PKCD, Protein kinase C delta type, Protein kinase C delta VIII, Protein kinase C, delta, Pkcdelta, PKC delta, Protein kinase c delta, Tyrosine-protein kinase PRKCD

Gene ID: 5580

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism