PKC zeta antibody (pThr410, Thr403)

Catalog No. ABIN229184

Product Details anti-PKC zeta Antibody

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Binding Specificity: pThr410, Thr403

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Detects endogenous human PKC zeta only when phosphorylated at Thr410, or endogenous human PKC lambda only when phosphorylated at Thr403. Does not cross-react with endogenous levels of other PKC isoforms. Species cross-reactivity: monkey, rat and mouse.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr410 of human PKC zeta.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PRKCZ / PKC-Zeta (PRKCZ Products)

Synonyms: TPKC antibody, PKCzeta antibody, prkcz-A antibody, pkc-zeta antibody, PRKCZ antibody, pkc2 antibody, PKC-ZETA antibody, PKC2 antibody, AI098070 antibody, C80388 antibody, Pkcz antibody, R74924 antibody, aPKCzeta antibody, zetaPKC antibody, 14-3-3-zetaisoform antibody, r14-3-3 antibody, pkcz antibody, si:ch211-150o23.4 antibody, protein kinase C zeta L homeolog antibody, protein kinase C zeta antibody, protein kinase C, zeta antibody, prkcz.L antibody, PRKCZ antibody, prkcz antibody, Prkcz antibody

Background: Name/Gene ID: PRKCZ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta

Gene ID: 5590

UniProt: Q05513

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling