14-3-3 sigma/SFN antibody

Catalog No. ABIN229459

This Mouse Monoclonal antibody specifically detects 14-3-3 sigma/SFN in WB, IHC, IF, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for 14-3-3 sigma/SFN antibody (ABIN229459)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 sigma/SFN Antibody

Specificity: Recognizes human 14-3-3 sigma protein (26-30kD). Does not cross-react with other isoforms of 14-3-3.

Purification: Protein G purified

Immunogen: Recombinant human 14-3-3 sigma protein (26-30kD). Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF, IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate. Use Protein G. Western Blot: 1 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: HT29 cells and skin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN / Stratifin / 14-3-3 Sigma

Background: Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction