BTK antibody (pSer180)

Catalog No. ABIN230892

The Mouse Monoclonal anti-BTK antibody (ABIN230892) specifically detects BTK in WB, ELISA and IP. The antibody is reactive with Human samples.

Quick Overview for BTK antibody (pSer180) (ABIN230892)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-BTK Antibody

Binding Specificity: pSer180

Specificity: Recognizes overexpressed levels of human Btk only when phosphorylated at Ser180 (Mr 77kD). This antibody cross-reacts with some other proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser180 of human Btk. Species Sequence Homology: Mouse - 100%.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IP (1:25), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. For Western Blot, USBio recommends using immunoprecipitated Btk. Immunoprecipitation: 1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 0.02 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: BTK

Background: Name/Gene ID: BTK
Subfamily: BTK/TEC
Family: Protein Kinase

Synonyms: BTK, AGMX1, AT, ATK, XLA, PSCTK1, B-cell progenitor kinase, BPK, Bruton tyrosine kinase, Tyrosine-protein kinase BTK, IMD1

Gene ID: 695

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling