CDK2 antibody (pThr160)

Catalog No. ABIN232740

This anti-CDK2 antibody is a Rabbit Polyclonal antibody detecting CDK2 in WB, ELISA and IP. Suitable for Human, Mouse and Rat.

Quick Overview for CDK2 antibody (pThr160) (ABIN232740)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CDK2 Antibody

Binding Specificity: pThr160

Specificity: Detects cdk2 only when phosphorylated at threonine 160. Weakly cross-reacts with cdc2 phosphorylated at Thr161. Does not cross-react with other phosphorylated cyclin-dependent kinases. Species cross-reactivity: Human, mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP (1:150), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:150.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: Name/Gene ID: CDK2
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK2, Cell division protein kinase 2, CDC2-related protein kinase, p33 protein kinase, p33(CDK2), CDKN2, Cyclin-dependent kinase 2

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA