IRS1 antibody (pSer612)

Catalog No. ABIN233249

The Rabbit Polyclonal anti-IRS1 antibody is suitable to detect IRS1 in samples from Mouse and Rat. It has been validated for WB and IP.

Quick Overview for IRS1 antibody (pSer612) (ABIN233249)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-IRS1 Antibody

Binding Specificity: pSer612

Specificity: Detects endogenous IRS-1 only when phosphorylated at serine 612 of mouse IRS-1 or serine 616 of human IRS-1. Does not detect IRS-1 phosphorylated at other sites. Species cross-reactivity: Human. Expected to cross-react with rat based on sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser612 of mouse IRS-1 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:50), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 / IRS-1

Background: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process